sCMOS Scientific Camera Successfully Realizes 20-Photon Single-Molecule Fluorescence Imaging

Single-molecule fluorescence imaging technology can accurately capture weak fluorescence signals within a single molecule, which is crucial for studying the real-time dynamic behavior of biological molecules and understanding the microscopic mechanisms of the basic units of life activities.

Single-molecule fluorescence imaging experiments have long been conducted based on EMCCD cameras, which have bottlenecks such as high cost, limited dynamic range, and low full- well capacity, which restrict the output of single-molecule research results.

Revealer's accurately grasps the needs and trends of scientific research, and develops a sCMOS scientific camera Gloria 1605 with large pixel size, high quantum efficiency (QE), high dynamic range, and large full well capacity . It has single -photon weak light detection capabilities, and has successfully achieved 20-photon signal detection in a university laboratory, and completed single-molecule sub-nanometer positioning accuracy verification.

The experimental conditions used a Gloria 1605 with a 16 μm pixel size, a 100X microscope, a 532 nm laser for excitation of single molecules, and the camera was adjusted to low-speed high-sensitivity mode. 1000 frames of images were collected continuously, and background fluorescence interference was eliminated through a real-time noise suppression algorithm. The single-molecule fluorescence image captured by sCMOS is as follows:

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The video shows that in a weak light environment with a detectable photon level of only 20 photons, the sCMOS camera successfully captured clear single-molecule fluorescence signals with a high QE of 90.7%.

The quality of fluorescence signals collected by sCMOS scientific cameras meets expectations. Experiments have strongly proved that sCMOS cameras have relative advantages in sensitivity, low noise, high dynamic range, and full well capacity. They can replace traditional EMCCDs, greatly reduce the cost of scientific research experiments, and become the preferred instrument for single-molecule fluorescence imaging research.

Attached is the performance parameter comparison between the sCMOS camera Gloria 1605 and a certain type of EMCCD camera

#sCMOS Scientific Cameras #Single-molecule fluorescence imaging #Scientific research equipment selection #EMCCD alternatives #Precision Optics